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Microscope Do The Necessary Cleaning And Adjustment Work
Edit: Ningbo Yaki Optical Instrument Co.,Ltd    Date: Jul 03, 2017

The use of the microscope steps

1, take the mirror and place it

① the right hand holding the mirror arm, left hand holding the mirror seat.

② the microscope on the bench, slightly left. Install eyepiece and objective lens.

2, the light

① Turn the converter so that the low magnification objective lens is aligned with the light hole. Note that the front end of the objective lens and the stage to keep 2 cm distance.

② to a larger aperture aligned through the light hole. Left eye gaze, the right eye opened, easy to observe after drawing. Turn the mirror and see the bright field of view.

3, observation

① to watch the slide to be observed on the stage, with the pressure clamp folder, the specimen should be on the light hole.

② turn the coarse focus screw, so that the tube slowly down until the objective lens close to the slide. Eyes looking at the objective lens to avoid the glass object.

③ the left eye to look inside the eyepiece, while the reverse rotation of the coarse quasi-focus spiral, so that the tube slowly rise until you see things like. And then slightly turn the fine coke spiral, so that the image is more clear.

1, before the preparation of observation

(1) set the microscope in a stable bench, the mirror seat from the edge of the experimental platform is about an inch or so. Specimen posture to correct, generally with the left eye observation, right eye easy to draw or record, two eyes must also open to reduce fatigue, can also practice the left and right eyes can be observed. The microscope is shown on the right.

(2) the microscope is an optical precision instruments, in use, be especially careful, familiar with the microscope before the structure and performance, check the total parts are intact. Whether the mirror body dust, the lens is clean, do the necessary cleaning and adjustment work.

(3) to adjust the light source should avoid direct light source, due to direct light source affect the image of the clear, damage the light source device and lens, and stimulate the eyes. Sunny day can be directly outside the window of scattered light, such as bright and dark weather, available 8-30W fluorescent lamp or microscope lighting

Adjust the light source and the general steps of light:

Rotate the lower objective lens to the bottom of the tube and rotate the coarse adjustment wheel so that the distance between the lens and the stage is about 0.5cm.

The rising condenser is as high as the stage of the stage. Otherwise use the oil mirror when the light is dark.

You can see the eyepiece, adjust the mirror mirror angle (the mirror has a concave side of both sides, strong light natural light source, it is appropriate to use a plane mirror; weak light natural light source or artificial light, it is appropriate to use concave mirror.) The light to the full field of view Uniform brightness. Where to check the stained specimens, the light should be strong; check unstained specimens, the light should not be too strong. Can be expanded or reduced by the aperture,

Lift the condenser, rotate the mirror to adjust the light.

2, low magnification observation

Inspect the specimen must first be observed with a low signal mirror, because the low magnification field of view is large, easy to find the target and determine the location of the inspection.

(1) Place the specimen slide on the stage and place the specimen under the objective lens. Turn the coarse adjustment wheel, the descending objective lens or the rising stage to make the objective lens at 0.5cm.

(2) the left eye to see the eyepiece, while anti-clockwise slowly rotating coarse adjustment wheel, when the visual field appears in the image, the use of fine tuning wheel, up and down slightly turn until the field of vision to obtain a clear image. And then carefully observe the various parts of the specimen, to determine and need to be further observed to move the central part of the field, ready to observe with high magnification.

3, high magnification observation

The high magnification to turn directly below, in the conversion of the lens, the need for eye observation in the side, to avoid the lens and slide collision. And then by the eyepiece observation, and then carefully adjust the aperture and condenser, so that the brightness of the light appropriate, and then carefully both positive and negative micro-rotation fine tuning wheel, until a clear image to find the most suitable for observation of the site. Need to be further

Observe the movement of the center of the field, ready to observe with the oil mirror.

4, oil mirror observation

(1) Rise of the condenser, full aperture iris

(2) with a coarse adjustment wheel lift tube or drop the stage, turn the converter to the oil mirror to the bottom of the tube. Drop a drop of asphalt in the mirror part of the specimen. The right hand slowly turn the coarse adjustment wheel clockwise to make the tube down or the stage is up, and at the same time, the oil mirror is immersed in the oil from the side of the microscope until it is almost in contact with the specimen. Not mind not

To pressure to the specimen, so as not to crush the slide, or even damage the oil lens.

(3) from the eyepiece to observe, to further adjust the light, so that the light is bright, and then the coarse adjustment wheel will slowly rise or slow down the stage until the visual field appears so far, and then fine adjustment wheel correction focal length The Such as the oil mirror has left the oil and still no image, must be observed from the side, the oil mirror down, repeat the operation to see the image so far.

5, for the film

After observing a specimen, if you want to observe another specimen, you must first turn the high magnification objective lens (or oil mirror) back to the low magnification objective lens, remove the specimen, according to the method of putting a new film, you can observe. Do not be in the high magnification objective lens (or oil actually mirror) under the film, to prevent damage to the lens.


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